FIGURE

Fig. 6

ID

ZDB-FIG-150331-20

Publication

Shao et al., 2009 - Down syndrome critical region protein 5 regulates membrane localization of Wnt receptors, Dishevelled stability and convergent extension in vertebrate embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Disruption of the membrane localization of Knypek and Frizzled 7 receptors in dscr5 morphants. (A) Localization of Kny-Myc at the cell surface and in the cytoplasm. (B) Kny-Myc distribution in dscr5-knockdown cells. (C) Western blot analysis of Kny-Myc levels in the culture medium (left) and cell pellets (right) of dscr5 RNA- or dscr5MO-injected cells. GFP protein was used as a loading control in the cell pellets and to monitor the integrity of the cultured cells. (D,E) XFz7-Myc localization revealed by Cy3-conjugated secondary antibody. (D) Localization of XFz7-Myc to the cell membrane of control cells. (E) XFz7-Myc distribution in dscr5-knockdown cells. (F) Caveolin-GFP localization in dscr5-knockdown cells. (G) Merge of E and F showing the colocalization between XFz7-Myc and Caveolin-GFP. (H-K) Rescue of XFz7-Myc membrane localization by dn-caveolin in cells injected with dscr5MO (I) or gly4ΔC RNA (K).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development