FIGURE

Fig. 1

ID

ZDB-FIG-150326-36

Publication

Dozawa et al., 2014 - Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum

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Fig. 1

VPA increases proliferating cells in the hindbrain of embryonic zebrafish. A: Embryonic zebrafish were treated with 1.0, 1.5, 2.0 mM VPA from 10 to 48 hpf. In last 20 min, BrdU was added to label proliferating cells. B: Number of BrdU-positive cells increased in a dose-dependent manner, and the area of BrdU-positive cells expanded in the VPA-treated brain. C: Number of BrdU-positive cells per section. D: Tg(elavl3:GFP) and Tg(gfap:GFP) were treated with VPA at indicated concentrations, and the GFP-positive area was analyzed. Reduction of elavl3-GFP-positive area (a-e, a′-e′) and increase of gfap-GFP-positive area (f-j, f′-j′) were observed in VPA-treated zebrafish hindbrain. E: In situ hybridization of VPA-treated embryonic brain with neurod probes. Area of neurod-positive decreased in VPA-treated zebrafish hindbrain. After whole-mount in situ hybridization, coronal sections were made at rhombomere 5 or 6 of the hindbrain.

Expression Data

Genes:	EGFP GFP neurod1
Fish:	WT knu3Tg mi2001Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	hindbrain neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: pharmaceutical
Observed In:	hindbrain cell population proliferation hindbrain neurogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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