FIGURE

Fig. 6

ID

ZDB-FIG-150304-29

Publication

Campbell et al., 2013 - Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Caspases-3 and -9 interact with Slit1a-Robo2 signaling to restrict arbor size. (A–L) Maximum intensity projections of 80-hpf RGC arbors in the optic tectum expressing YFP-Rab3. WT (A), coMO (B), casp-3aMO1 (C), casp-9MO1 (D), slit1a transMO1 (low dose; E), ast/+ (F), robo2SBMO1 (low dose; G), slit1a transMO1 (low dose); casp-3aMO1 (H), slit1a transMO1 (low dose; I), ast (J), ast/+; casp-3aMO1 (K), and robo2SBMO1 (low dose); casp-9MO1 (L) are shown. (M–R) Quantification of arbor parameters: branch tip number (M), arbor area (N), total arbor length (O), arbor order (P), presynaptic puncta number (Q), and puncta density (R). 7–15 arbors were analyzed per condition. Part of the ast data is obtained from Campbell et al. (2007). Dorsal views are shown, and anterior is up. White asterisks indicate the parent axon. Error bars represent SEMs. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	robo2^{ti272z/ti272z} WT + MO1-casp3a WT + MO1-slit1a WT + MO2-casp9 WT + MO3-robo2 robo2^ti272z/+ robo2^ti272z/+ + MO1-casp3a WT + MO1-casp3a + MO1-slit1a WT + MO1-slit1a + MO2-casp9 WT + MO2-casp9 + MO3-robo2
Knockdown Reagents:	MO1-casp3a MO1-slit1a MO2-casp9 MO3-robo2
Observed In:	retinal ganglion cell axon retinal ganglion cell axon collateral retinal ganglion cell collateral sprouting in absence of injury retinal ganglion cell presynaptic active zone
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.