FIGURE

Fig. 3

ID

ZDB-FIG-150130-10

Publication

Essers et al., 2014 - A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis

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Fig. 3

Specific rRNA processing defects are evident in ns and gnl2 mutants. (A) Simplified schematic of rRNA processing. The locations of the probes used and the processing intermediates visualized in (B) are indicated. (B) Northern blots showing reduced cleavage activity in various rRNA processing steps in gnl2, ns and gnl3l mutants at 2 dpf. The arrowheads indicate sites of major processing defects in the mutants. Results are representative of at least three independent experiments. (C) Relative band intensities were measured using ImageJ software and are represented relative to the wild type sibling for each mutant. p<0.01 by Student′s two-tailed t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gnl2^bw41c/bw41c gnl3^{hu3259/hu3259} gnl3l^{hi1437Tg/hi1437Tg}
Observed In:	cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 385(2), Essers, P.B., Pereboom, T.C., Goos, Y.J., Paridaen, J.T., and Macinnes, A.W., A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis, 304-15, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.