FIGURE

Fig. 1

ID

ZDB-FIG-150129-9

Publication

Zhao et al., 2014 - Leptin and IL-6 Family Cytokines Synergize to Stimulate Müller Glia Reprogramming and Retina Regeneration

Other Figures

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Fig. 1

The Jak/Stat3 Signaling Pathway Is Activated following Retinal Injury

(A) Immunofluorescence on retinal sections shows activated p-Stat3 expression in BrdU⁺ MG-derived progenitors that are localized to the injury site at 4 dpi.

(B) A schematic of the gfap:stat3-gfp transgene construct shows the fusion gene, stat3-gfp, under control of the gfap promoter regulatory elements.

(C) In gfap:stat3-gfp transgenic fish, Stat3-GFP fusion protein expression is undetectable in MG of the uninjured eye and is restricted to MG-derived progenitors at the injury site at 4 dpi. White dots indicate autofluorescence unique to the green channel (see Figure S2G).

(D) Confocal images show colocalization of Stat3-GFP with GS⁺/p-Stat3⁺/BrdU⁺ MG-derived progenitors at 4 dpi.

In (A) and (C), the asterisk marks the injury site (needle poke) and arrows point to MG-derived progenitors. Scale bars, 50 µm (A and C) and 20 µm (D). INL, inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium; dpi, days postinjury. See also Figures S1 and S2.

Expression Data

Gene:	EGFP
Antibody:	Ab1-stat3
Fish:	WT mi35Tg
Condition:	physical alteration: retina
Anatomical Term:	Muller cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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