FIGURE

Fig. 3

ID

ZDB-FIG-141125-10

Publication

Hartwig et al., 2014 - Temporal control over the initiation of cell motility by a regulator of G-protein signaling

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Fig. 3

The acquisition of cell motility is inhibited by Rgs14a. (A) Snapshots from time-lapse movies showing PGC migration-onset events (mpf = minutes post fertilization). Rgs14a morphants (MO1) exhibit earlier PGC migration onset events (white arrows) compared with control morphants (Movie S1). (B) PGC migration onset events for Rgs14a and control morphants, as well as Rgs14a overexpression. Knockdown of Rgs14a leads to premature migration onset, whereas overexpression of 100 pg rgs14a-globin32UTR delays PGC migration onset. Combination of both treatments restored WT motility acquisition timing. Time point zero is defined as the PGC specification event at 180 mpf (9). pa(photoactivatable)-gfp-globin32UTR was used as a control mRNA. n = number of cells analyzed. (B) ***P < 0.001 using Mann–Whitney u test. Error bars indicate SEM.

Expression Data

Gene:	EGFP
Fish:	er1Tg er1Tg + MO1-rgs14a
Knockdown Reagent:	MO1-rgs14a
Anatomical Term:	primordial germ cell
Stage Range:	High to Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	er1Tg + MO1-rgs14a
Knockdown Reagent:	MO1-rgs14a
Observed In:	germ cell migration primordial germ cell
Stage Range:	High to Shield

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA