FIGURE

Fig. 3

ID

ZDB-FIG-141112-19

Publication

Park et al., 2014 - A Single Mutation in the Acetylcholine Receptor δ-Subunit Causes Distinct Effects in Two Types of Neuromuscular Synapses

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Fig. 3

Different distribution of AChRs in slow and fast muscle fibers. A, Cross section of a wild-type trunk stained with the F59 antibody. The displayed region is indicated in E. F59-positive slow muscle cells form a single layer at the most superficial region. B, C, Optical slices of a wild-type larva near the surface (B) or deeper (C). Magenta represents α-BTX staining; green represents the F59 antibody signal. Scale bar, 50 μm. D, Stochastic expression of wild-type δ subunit in the sofa potato mutant. A slow muscle that expresses the transgene displayed cytoplasmic GFP (arrow; green) as well as α-BTX-positive AChR (arrowhead; magenta). Because fast muscles in the neighboring layer lacked the transgene expression, only myoseptal α-BTX staining was observed. Scale bar, 50 μm. E, Schematic cross section of the trunk of a larval zebrafish. Slow muscles are marked with green. Optical sections corresponding to A-C are indicated with a box and planes. SC, Spinal cord; NC, notochord.

Expression Data

Gene:	chrna1
Antibody:	Ab-F59
Fish:	AB/TU
Anatomical Terms:	fast muscle cell acetylcholine-gated channel complex fast muscle cell medial region slow muscle cell slow muscle cell acetylcholine-gated channel complex slow muscle cell anterior margin slow muscle cell posterior margin
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.