A) Select images from a time lapse video of 4 dpf FoxD3-/-;Ntr treated with 10 mM Met for 18 h. Puncta around plexus (arrows) and red motile cells (arrowheads) appeared around 10 h after start of Met treatment. For comparison, wild type Ntr larvae developed puncta after 2–4 hr in Met (Figure 1B′). B) Quantitation of cell death in 4 dpf Met-treated FoxD3-/-Ntr compared to untreated FoxD3-/-;Ntr. AO-stained cell counts after various incubation times in 10 mM Met. There was a significant increase in cell death after 2.5 hour Met incubation (control 18.00±4.96, n = 9 and Met 59.06±6.66, n = 16, p<0.05), but not after 4 h (control 16.0±3.1 cells, n = 5 and Met 47.3±12.3, n = 6, ns) or 5 h (control 26.6±6.0 cells, n = 5 and Met 37.6±11.0, n = 5, ns) Met treatment. For all panels, anterior is to the left; dorsal is at the top. Eye and otic vesicle (OV) are outlined. Cranial ganglia labeled gV, gVII, gIX and gX in panel A.
