FIGURE

Fig. 5

ID

ZDB-FIG-140908-5

Publication

Benato et al., 2014 - A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish

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Fig. 5

Reduced GRE activity following gr MOs injections alone or combined with 10 µM DEX treatment of 2-dpf transgenics for 24 h. Top panel: EGFP protein localization in control (ia20 embryos) and after translation-blocking MO (gr^ATG1MO), missplicing MO (gr^splicMO) and mismatched control MO (gr^mismMO) injections alone (on the left) or combined with DEX treatment (on the right). Middle and bottom panels: fold changes in gene expression of fkbp5 and egfp in embryos injected with gr^mismMO, gr^ATG1MO and gr^splicMO w/wo DEX treatment as compared to non- injected/non-treated control (set at 1). The expression levels of the target genes were normalized on ef1a as housekeeping gene. Values represent the mean ± S.E. Asterisks indicate expression levels that are significantly different from control (ia20 embryos) or between samples as indicated by the horizontal line: **P < 0.01; ***P < 0.001. Scale bar: 200 µM.

Expression Data

Gene:	EGFP
Fish:	ia20Tg ia20Tg + MO2-nr3c1 ia20Tg + MO4-nr3c1
Condition:	chemical treatment: pharmaceutical
Knockdown Reagents:	MO2-nr3c1 MO4-nr3c1
Anatomical Terms:	head whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 392(1-2), Benato, F., Colletti, E., Skobo, T., Moro, E., Colombo, L., Argenton, F., Dalla Valle, L., A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish, 60-72, Copyright (2014) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.