Fig. 2

Mtp mediates BODIPY^® FL C₁₂ absorption. BODIPY^® FL C12 (1.5 ng/nl) was injected into the yolk of 1.5-dpf larvae obtained from adult mtp heterozygote (mtp^+/-) crosses. Following injection, larvae were incubated for 3 hours and then analyzed for vascular fluorescence. mtp mutants were identified phenotypically, as described previously (Avraham-Davidi et al., 2012). The images shown are representative for (A) wild-type and/or heterozygous siblings and (B) mtp-mutant larvae. Nomarski images are shown in the left panels. Fluorescent images (right panels) reveal that BODIPY^® FL C₁₂ was distributed in the tail vasculature of wild-type and mtp^+/- larvae (A), whereas mtp-mutant larvae (B) have negligible levels of vascular BODIPY-labeled fatty acid fluorescence. (C) Circulating BODIPY^® FL C₁₂ fluorescence was quantified (shown in the right-hand panel) in the caudal artery and veins (area 1). A background correction was performed using a non-vascularized region (area 2). Four experiments were performed using 21–24 larvae per experiment. Data are represented as the mean of means±the pooled s.e.m., ***P<0.00001, Student’s t-test.