FIGURE

Fig. 5

ID

ZDB-FIG-140613-27

Publication

Dickover et al., 2014 - The atypical Rho GTPase, RhoU, regulates cell-adhesion molecules during cardiac morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Arhgef7b is required to localize N-cadherin and Alcama to the cell junctions. (A, E) Brightfield microscopy showed that (E) the arhgef7b mutant, bubblehead (bbh), exhibited cardiac edema (red arrow) and cranial hemorrhaging (asterisk) by 60 hpf. (B, F) Confocal projections of 60 hpf control wild-type sibling and bbh mutant phalloidin stained (F-actin) hearts revealed that bbh mutant hearts failed to undergo proper cardiac looping and displayed AV canal defects. White arrowhead points to AV canal. (C, D, G, H) Immunohistochemical staining of control and bbh mutant hearts at 60 hpf using (C, G) Alcama and (D, H) N-Cadherin antibodies, revealed mislocalization of Alcama and N-cadherin in bbh mutant AV cardiomyocytes (G, H – white arrow). At – atrium, V – ventricle. (I) bbh/arhgef7b genetically interacts with rhoua. Cardiac edema and AV canal defects were observed more frequently in bbh +/ embryos than in wild-type embryos upon increasing titrated doses of rhoua MO1. (WT+2.5, 5, 7.5, 10 ng MO1 – 0%, 21.4%, 52.7%, 88.7% cardiac phenotype and arhgef7b^+/m292+2.5, 5, 7.5, 10 ng MO1 – 15.4%, 37.5%, 78.9%, 100% cardiac phenotype).

Expression Data

Antibodies:	Ab1-cdh2 zn-8
Fish:	WT arhgef7b^m292/m292
Anatomical Terms:	atrioventricular canal cardiac muscle cell atrioventricular canal cell junction atrioventricular canal cytoplasm
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	arhgef7b^m292/m292 arhgef7b^m292/+ + MO1-rhoua
Knockdown Reagent:	MO1-rhoua
Observed In:	atrioventricular canal brain heart heart looping
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 389, Dickover, M., Hegarty, J.M., Ly, K., Lopez, D., Yang, H., Zhang, R., Tedeschi, N., Hsiai, T.K., Chi, N.C., The atypical Rho GTPase, RhoU, regulates cell-adhesion molecules during cardiac morphogenesis, 182-91, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.