Fig. 2

Diamines elicit c-Fos and pERK increase in olfactory sensory neurons. (A) Zebrafish (n = 21) were exposed to stimuli indicated (2 or 5 mM). c-Fos IHC (green) and nuclear staining (propidium iodide, red), enabled visualization of c-Fos⁺ nuclei (yellow), some emphasized by gray arrow heads. (B and C) Quantification of c-Fos⁺ nuclei/lamella as a function of diamine chain length (B) or concentration (C). Results from one representative experiment each are shown. Counting was done on randomized micrographs, values given represent mean ± SEM. Significance in comparison with water was evaluated by Student t test, *P < 0.05, **P < 0.01. (B) 1, water; 2, food extract; 3–8, numbers reflect carbon chain length of diamines; 9, diaminodecane. (D) Zebrafish (n = 14) were exposed to stimuli indicated (1 mM). Some pERK-labeled cells (red) are emphasized by gray arrowheads; nuclear counterstain (DAPI, blue). Red central stripes in some panels, unspecific label in the basal lamina outside the sensory region. (E and F) Quantification of pERK⁺ cells/lamella as function of chain length (E) or concentration (F). Values given represent mean ± SEM. Significance in comparison with water was evaluated by Student t test, **P < 0.01. (E) Results from two experiments are shown; 1, water; 2, food extract; 3–8, numbers reflect carbon chain length of diamines; 9, diaminodecane. (F) Evaluation was partly on randomized data, no difference was seen between randomized and nonrandomized evaluation.