FIGURE

Fig. 6

ID

ZDB-FIG-140522-11

Publication

Nissim et al., 2014 - Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth

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Fig. 6

PGE₂ Interacts with the bmp2b Pathway to Regulate Liver versus Pancreas Specification

(A) Modulation of PGE₂ levels from 12 to 20 hpf impacts expression of bmp2b in the lateral plate mesoderm as shown by in situ hybridization at 20 hpf. dmPGE₂ increases bmp2b expression (n = 15/19 embryos), whereas Indo decreases bmp2b expression (n = 14/21 embryos). Lower: magnified view of the area in the dashed square. bmp2b is also expressed in the otic vesicles (arrow).

(B and C) Size of pancreas-specified population marked by pdx1 is impacted by modulation of the PGE₂ or bmp2b pathways. Representative views are shown in (B), and quantification is shown in (C). dmPGE₂ or heat-shock activation of bmp2b diminishes whereas Indo, dorsomorphin (Dorso), or heat-shock activation of dnBmpR promotes the pancreas progenitor population. The impact of dmPGE₂ is blunted by Dorso or dnBmpR, and the impact of Indo is blunted by heat-shock activation of bmp2b. (Data are represented as mean ± SEM; n e 10 per condition; t test comparisons: p < 0.05 comparing treatment versus control; ^#no significant difference comparing with versus without dmPGE₂; ^##no significant difference comparing with versus without Indo.)

Expression Data

Genes:	bmp2b pdx1
Fish:	WT fr13Tg w30Tg
Conditions:	chemical treatment: prostaglandin E2 chemical treatment: dorsomorphin chemical treatment: dorsomorphin chemical treatment: dorsomorphin chemical treatment: indometacin heat shock chemical treatment: prostaglandin E2, heat shock chemical treatment: indometacin, heat shock
Anatomical Terms:	lateral plate mesoderm pancreas primordium
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT fr13Tg w30Tg
Conditions:	chemical treatment: prostaglandin E2 chemical treatment: dorsomorphin chemical treatment: dorsomorphin chemical treatment: dorsomorphin chemical treatment: indometacin heat shock chemical treatment: prostaglandin E2, heat shock chemical treatment: indometacin, heat shock
Observed In:	lateral plate mesoderm pancreas primordium
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 28(4), Nissim, S., Sherwood, R.I., Wucherpfennig, J., Saunders, D., Harris, J.M., Esain, V., Carroll, K.J., Frechette, G.M., Kim, A.J., Hwang, K.L., Cutting, C.C., Elledge, S., North, T.E., and Goessling, W., Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth, 423-437, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell