FIGURE

Fig. S6

ID

ZDB-FIG-140515-36

Publication

Le Guen et al., 2014 - Ccbe1 regulates Vegfc-mediated induction of Vegfr3 signaling during embryonic lymphangiogenesis

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Fig. S6

Tg(shh:vegfc) partly rescues lymphatic development in ccbe1^hu3613 mutants but not in ccbe1 morphants. (A) Confocal projections in the Tg(flt4:YFP) background. In wildtype embryos PLs are visible at 3.5dpf (arrows) and TD is present at 5dpf (arrow heads), but not in ccbe1^hu3613 mutants, PLs and TD are missing (asterisks). However upon vegfc overexpression in the Tg(shh:vegfc) some PLs (arrows) and TD fragments (arrow heads) are observed in ccbe1^hu3613 mutants. (B) Quantitation of PLs and TD rescue in the Tg(shh:vegfc) in the absence of ccbe1. When vegfc is ectopically expressed in the floorplate, more than 70% of ccbe1^hu3613 mutants have PLs, and 55% develop TD fragments. (C) Confocal projections in the Tg(flt4:YFP) background. In wildtype embryos PLs are visible at 3.5dpf (arrows), but not in ccbe1 morphants (asterisks). Upon vegfc overexpression in the Tg(shh:vegfc) PLs (asterisks) are not rescued in ccbe1 morphants. (D) Quantitation of PLs rescue in the Tg(shh:vegfc) in wildtypes and ccbe1 morphants. When vegfc is ectopically expressed in the floorplate, 0% of ccbe1 morphants have PLs. (E) In the Tg(hsp70l:Gal4;4xUAS:vegfc) line no PL or TD rescue was observed after ccbe1 morpholino injection.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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