FIGURE

Fig. 6

ID

ZDB-FIG-140506-11

Publication

Dash et al., 2014 - sept7b is essential for pronephric function and development of left-right asymmetry in zebrafish embryogenesis

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Fig. 6

Apical actin is reduced in the pronephric tubule epithelia of sept7b-knockdown larvae. (A,B) Staining with phalloidin shows prominent apical actin (arrows) in the pronephric tubule of larvae injected with the TBMO-MM control (ctrl; A). By contrast, the intensity of actin staining is reduced in larvae injected with the sept7b TBMO (TBMO, B). The arrowheads point to muscle with intense labeling for actin. DAPI (blue) stains the nuclei. (C) The fluorescence intensity of actin staining in the pronephric tubules is reduced in sept7b morphants. The same settings were used during imaging of control and morphant larvae, and the images were analyzed without processing. Scale bars: 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-septin15
Knockdown Reagent:	MO1-septin15
Observed In:	pronephric tubule actin cytoskeleton pronephric tubule apical part of cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.