FIGURE

Fig. 6

ID

ZDB-FIG-140416-44

Publication

Viringipurampeer et al., 2014 - Rip3 knockdown rescues photoreceptor cell death in blind pde6c zebrafish

Other Figures

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Fig. 6

Phenotype at 4d.p.f. after morpholino knockdown of rip3 in wild-type (wt) and pde6c^w59 mutant (mt) embryos. (a–d) rip3 morpholino (MO) injection. (e–g) rip3 mismatch morpholino (MM) injection. (a and e) Control injections of MO or MM in wt embryos. Zpr1 cone-specific labelling in red. Scale bar=μm. (b) rip3 MO knockdown in mt embryos showing increased zpr1 labelling in red. (c) TUNEL labelling in rip3 MO knockdown mt embryos. (d) TUNEL labelling in mt embryos co-injected with rip3 MO and rip3 RNA. (f) rip3 MM knockdown in mt embryos showing decreased zpr1 labelling in red. (g) TUNEL labelling in mt embryos injected with rip3 MM. (h) ELISA quantitation of carbonyl content in wt, mt and MO-injected embryos at 4d.p.f. (white bars) or 7d.p.f. (black bars). Mean±S.E.M., n=8, *P<0.0001; **P<0.001

Expression Data

Antibody:	zpr-1
Fish:	WT pde6c^w59/w59 WT + MO2-ripk3 pde6c^w59/w59 + MO2-ripk3
Knockdown Reagent:	MO2-ripk3
Anatomical Terms:	green sensitive photoreceptor cell red sensitive photoreceptor cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pde6c^w59/w59
Observed In:	retinal cone cell retinal cone cell cell death
Stage:	Day 4

Phenotype Detail

Acknowledgments

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