FIGURE

Fig. S1

ID

ZDB-FIG-140325-18

Publication

Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number

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Fig. S1

Expression patterns of tagln2 and pdgfrb in zebrafish embryos and larvae. (A-C) ISH for tagln2. (A,B) tagln2 expression in trunk vessels at 48 hpf and 72 hpf, respectively. Arrowhead, tagln2+ putative mural cell in ISV. (C,D) tagln2 expression in brain vasculature at 48 hpf and 72 hpf. Arrow, tagln2+ putative brain mural cell. Inset, high magnification view of tagln2+ cells in the brain. (E-L) desmina, desminb, rgs5a, and cspg4 expression in trunk and brain vasculature at 72 hpf. (E,G,I,K) trunk expression. (F,H,J,L) brain expression. (M) Fluorescent ISH showing pdgfrb expression in retinal pericytes at 72 hpf. Arrowheads, pdgfrb+ retinal pericytes. (N) Quantification of pdgfrb+ pericytes associated with different hindbrain vessels at various time points. (O,P) foxd3 MO knockdown caused reduction in pdgfrb+ brain pericyte number. (O) Control MO-injected 72 hpf larva. (P) foxd3 MO-injected 72 hpf larva. Arrowheads, pdgfrb+ brain pericytes. HA, hyaloid artery; HV, hyaloid vessels. Scale bars: (A-L) 0.5 mm, (M-P) 200 μm.

Expression Data

Genes:	cspg4 desma desmb EGFP pdgfrb rgs5a tagln2
Fish:	WT y1Tg y1Tg + MO2-foxd3 + MO3-foxd3
Knockdown Reagents:	MO2-foxd3 MO3-foxd3
Anatomical Terms:	cranial blood vessel cranial blood vessel pericyte hyaloid vessel hyaloid vessel pericyte intersegmental vessel intersegmental vessel pericyte optic artery optic artery pericyte trunk vasculature
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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