FIGURE

Fig. 6

ID

ZDB-FIG-140311-38

Publication

Musso et al., 2014 - Novel cardiovascular gene functions revealed via systematic phenotype prediction in zebrafish

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Fig. 6

Expression analysis confirms the lack of a systematic developmental delay following tmem88a knockdown. Markers specific to the endothelium (fli1), developing muscle (myoD) and hematopoiesis (beta-globin E3) were visualized using in situ hybridization at 24 (A-I), 48 (J-R) and 72 (S-AA) hpf in wild-type embryos (A-C,J-L,S-U) and following injection of either the ATG (D-F,M-O,V-X) or splice-blocking (G-I,P-R,Y-AA) tmem88a morpholinos. Whereas fli1 and myoD expression appears unaltered, beta-globin E3 shows a slight elevation in expression following tmem88a knockdown at 48 hpf, which is largely alleviated by 72 hpf. High-resolution images of any panel are available upon request.

Expression Data

Genes:	fli1 hbbe3 myod1
Fish:	WT WT + MO1-tmem88a WT + MO2-tmem88a
Knockdown Reagents:	MO1-tmem88a MO2-tmem88a
Anatomical Terms:	endothelial cell hematopoietic system muscle
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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