Expression of nk2.2, shh/vhh-1 and axial in homozygous mutant cyclops embryos. Lateral views (A,D,E,F) with rostral to the left, and transverse sections (B,C) of embryos hybridised with nk2.2, axial or shh/vhh-1 anti-sense RNA. (A-C) nk2.2 expression in 18 somite (18h) wild-type and cyclops mutant embryos. The transverse sections shown in B and C are at the level of the diencephalon. The small dark patch on the dorsal surface of the embryo in B is an artefact. (D) 30 somites (24h) cyclops mutant embryos hybridised with antisense RNA to nk2.2, axial and shh/vhh-1. The arrowheads indicate a small cluster of axial and shh/vhh-1-expressing cells in the forebrain. (E) shh/vhh-1 expression at the dorsal tip of the mid-diencephalic furrow of a 30 somite (24h) cyclops mutant embryo. (F) nk2.2 expression at the tip of the middiencephalic furrow of a 40-44h cyclops mutant embryo. Abbreviations: cb, cerebellum; e, epiphysis; fe, fused eye; l, lens; mdb, middiencephalic boundary; mdf, mid-diencephalic furrow; ov, optic vesicle; t, telencephalon; te, tectum. Scale bar=100 μm.
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