FIGURE

Fig. 6

ID

ZDB-FIG-140214-26

Publication

Dheen et al., 1999 - Zebrafish tbx-c functions during formation of midline structures

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Analysis of tbx-c expression and effect of ectopic expression of tbx-c+ in mutants. (A,B) Expression of tbx-c in wild-type (A) and ntl^-/- mutant (B) embryos. Note the segregation of axial mesoderm (arrowheads) and a low level expression of tbx-c in the midline at the trunk of the mutant (arrows). (C-E) Co-localization of ehh (blue) and Ntl (brown) in wild-type (C), tbx-c+ injected ntl^-/- (D) and normal sibling(E) embryos obtained by crossing heterozygous flh^-/+ pair. Expression of both ehh and Ntl is expanded in the tbx-c+ injected wild-type sibling embryo (E) and absent in the tbx-c+ injected ntl^-/- mutant embryo (D). (F) tbx-c expression in the flh^-/- embryo. The notochord is absent and tbx-c is undetectable in the midline region of flh^-/-embryo. (G,H) ntl expression in the flh^-/- embryo (G) and the flh^-/- embryo injected with tbx-c+ (H). A few ntl positive cells (arrows) can be seen in the midline of both group of embryos. (I,J) tbx-c expression in wild-type (I) and spt^-/- embryos (J) at 14 hpf. Arrows indicate the tbx-c expression in the notochord. Insert (C,E): high magnification to show co-expression of ehh and Ntl in the notochord. Scale bars, 100 μm.

Expression Data

Genes:	ihhb tbx2b tbxta
Antibody:	Ab1-ta
Fish:	WT noto^n1/n1 tbx16^b104/b104 tbxta^b160/b160
Anatomical Terms:	axial mesoderm notochord
Stage Range:	1-4 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	noto^n1/n1 tbx16^b104/b104
Observed In:	notochord
Stage Range:	1-4 somites to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development