FIGURE

Fig. 2

ID

ZDB-FIG-140213-2

Publication

Wu et al., 2014 - Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development

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Fig. 2

Photoreceptors and projection neurons are not maintained in the pineal complex of med12 mutants. Rod photoreceptors were absent at 4 dpf in med12 mutants, as labeled by immunofluorescence for 4c12 (A, A′) and Opsin1 (B, B′). Cone cells are also absent in med12 mutants at 4 dpf, as shown by Arr3a staining (C, C′). Elavl3 staining showed a reduction in the number of the projection neurons at 4 dpf (D, D′). med12 mutants and WT showed comparable expression of the TgBAC[flh:kaede] transgene (E, E′), which labels the pineal complex at 1 dpf. Cell nuclei are stained with ToPro3 (blue).

Expression Data

Genes:	GFP Kaede
Antibodies:	Ab-4C12 Ab1-elavl Ab8-rho zpr-1
Fish:	zf104Tg vu376Tg med12^vu304/vu304; zf104Tg med12^vu304/vu304; vu376Tg
Anatomical Terms:	pineal complex pineal complex neuron pineal complex photoreceptor cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med12^vu304/vu304; vu376Tg
Observed In:	photoreceptor cell pineal complex
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 385(1), Wu, S.Y., de Borsetti, N.H., Bain, E.J., Bulow, C.R., and Gamse, J.T., Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development, 13-22, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.