Fig. S1

Phenotype analysis of eif3ba mutant at later developmental stages and expression pattern analysis of eif3ba. (A-B) At ~2 dpf, the mutant embryo (B) exhibits visible defects including delayed pigmentation, craniofacial malformation, and intumescent pericardial cavity and venous sinus. (C-D′) Heart morphology revealed by crossing the eif3ba mutation with Et(gata2a:EGFP)^pku3, a transgenic fish line with its EGFP reporter gene recapitulating the expression pattern of nppa. At 2 dpf, the wild-type heart (C, C′) has completely finished looping, while the heart of the mutant (D, D′) is still prolonged due to incomplete looping. (E) RT-PCR results of eif3ba in wild-type and mutant embryos. The mRNA level is dramatically reduced in the mutant, and only a faint longer strip (red arrow) containing the 595-bp RV sequence is detectable. (F-I′) In situ hybridization results of eif3ba in zebrafish embryos. The eif3ba mRNA is expressed throughout the embryo from the 1-cell stage to 16 ss. (J-L′) The expression pattern of eif3ba from 20 ss to 48 hpf. The cranial region stains darker at 20 ss (J) and 24 hpf (K) than the trunk. Particularly, at 24 hpf eif3ba is strongly expressed in the branchial primordia (K′, red arrows), which are thought to be in the cranial NCC migration pathway. At 48 hpf, eif3ba is expressed in the brain (L, L′). WT, wild type. (A-D, G-L) Lateral view with anterior to the left and dorsal up; (G′-L′) dorsal view with anterior to the left; (C′-D′) ventral view.