FIGURE

Fig. 7

ID

ZDB-FIG-131213-30

Publication

Schumacher et al., 2013 - tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium

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Fig. 7

Myocardial genes are ectopically expressed in the tal1-deficient endocardium at 60 hpf. (A–D) Three-dimensional reconstructions of confocal stacks (A and C) and single optical sections through the ventricle (B and D) depict wild-type (A and B) and tal1-deficient hearts (C and D) expressing Tg(myl7:H2A-mCherry) (red) and Tg(fli1a:negfp) (green) at 60 hpf, arterial pole at the top. Many tal1-deficient endocardial cells express both Tg(myl7:H2A-mCherry) and Tg(fli1a:negfp) (D), but wild-type endocardial cells express only Tg(fli1a:negfp) (B). (E and F) Immunofluorescence with the monoclonal antibody MF20 detects sarcomeric myosin heavy chain localization (blue) in wild-type (E) and tal1-deficient hearts (F) expressing Tg(myl7:H2A-mCherry) (red) and Tg(fli1a:negfp) (green). Optical sections through the ventricle reveal ectopic sarcomeric myosin heavy chain within the tal1-deficient endocardium (F). In contrast, sarcomeric myosin heavy chain is not found in the wild-type endocardium (E); section shown depicts both the atrial endocardium (bottom) and outflow tract endocardium (top). (B′, D′, and F′) Images display only the myocardial markers from the sections shown in (B, D, and F).

Expression Data

Genes:	EGFP mCherry
Antibody:	Ab-MF20
Fish:	sd12Tg; y7Tg (AB) sd12Tg; y7Tg + MO5-tal1 + MO6-tal1 (AB)
Knockdown Reagents:	MO5-tal1 MO6-tal1
Anatomical Terms:	endocardium myocardium
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sd12Tg; y7Tg + MO5-tal1 + MO6-tal1 (AB)
Knockdown Reagents:	MO5-tal1 MO6-tal1
Observed In:	endocardium cardiac muscle cell differentiation
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 383(2), Schumacher, J.A., Bloomekatz, J., Garavito-Aguilar, Z.V., and Yelon, D., tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium, 214-226, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.