Fig. 1

Effectiveness of RPS19 morpholinos and hemoglobin staining of embryos coinjected with Rps19 mRNA and P53 MO using o-dianisidine.

The Rps19:egfp construct was made by inserting a partial sequence of Rps19 cDNA (containing 60 bps from the 52 UTR ) with the N-terminus of egfp into modified pEGFP-N1 (the ATG codon of EGFP was removed). The sequence of RPS19 MO1 is a compliment of bp 1–24 of Rps19 cDNA. Embryos co-injected with 25 ng Rps19:egfp DNA and 5 ng control MO produced green fluorescent fusion protein (A), and expression of the fusion protein was inhibited by co-injection with 2 ng Rps19 Mo (B). O-staining results show a drastic reduction in the number of hemoglobin-stained blood cells when Rps19 is knockdown (C and D are the control, E and F are Rps19 knockdown) and partially reversed by co-injection of P53 morpholino (G and H). A, B, D, F and H are the lateral view; C, E and G are the ventral view.