FIGURE

Fig. 2

ID

ZDB-FIG-130907-17

Publication

Laux et al., 2013 - Circulating Bmp10 acts through endothelial Alk1 to mediate flow-dependent arterial quiescence

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Fig. 2

Alk1 activity is dependent on blood flow. (A-E) pSmad1/5/9 (middle column) in endothelial cell nuclei (marked by fli1a:negfp transgene, left column); in merge (right column), EGFP-expressing endothelial cell nuclei are green and pSmad1/5/9 immunofluorescence is magenta. (A) 24 hpf wild type, prior to blood flow; (B) 36 hpf tnnt2a morphant (no flow); (C) 36 hpf wild type; (D) 36 hpf tnnt2a morphant harboring a fli1a:alk1-myc transgene; (E) 36 hpf tnnt2a morphant harboring a fli1a:alk1^CA-mCh transgene. Tg(fli1a:alk1^CA-mCh) embryos do not have lumenized vessels. In merge (right column), EGFP-expressing endothelial cell nuclei are green, pSmad1/5/9 immunofluorescence is magenta. Yellow and blue arrows indicate endothelial cells in the caudal division of the internal carotid artery (CaDI) and basal communicating artery (BCA), respectively. 2D confocal projections of 50 μm frontal sections, dorsal upwards. Scale bar: 50 μm. See supplementary material Table S1 for fluorescence quantitation.

Expression Data

Gene:	EGFP
Antibody:	Ab1-smad
Fish:	y7Tg y7Tg + MO1-tnnt2a pt516Tg; y7Tg + MO1-tnnt2a
Knockdown Reagent:	MO1-tnnt2a
Anatomical Terms:	basal communicating artery endothelial cell caudal division of the internal carotid artery endothelial cell endothelial cell nucleus
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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