FIGURE

Fig. 3

ID

ZDB-FIG-130906-3

Publication

Forbes-Osborne et al., 2013 - Insulinoma-associated 1a (Insm1a) is required for photoreceptor differentiation in the zebrafish retina

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Fig. 3

Photoreceptor cell differentiation is impaired in insm1a morphants. (A) At 3 dpf cone (green) and rod (red) photoreceptor cells were severely reduced in insm1a morphants. (B) The reduction in rods was significant and could be partially rescued by co-injection of in vitro transcribed insm1a mRNA with the insm1a morpholino (control: n=31 eyes from 22 embryos; insm1a MO: n=24 eyes from 17 embryos; insm1a MO+insm1a mRNA: n=18 eyes from 10 embryos). (C) The reduction in cones was significant and could be rescued by co-injection of in vitro transcribed insm1a mRNA with the insm1a morpholino (control: n=26 eyes from 17 embryos; insm1a MO: n=24 eyes from 17 embryos; insm1a MO+ insm1a mRNA n=18 eyes from 10 embryos). (D) At 4 dpf, the rod photoreceptors remain significantly reduced. However, cone photoreceptors (green) significantly recovered, but were less dense and contained visible gaps (dotted box). (D2) In regions lacking cones INL cells breached the OPL and extended into the ONL. Both small (arrowheads) and large (arrows) gaps were observed, accompanying the breached OPL. (B, D: ⋄=mean, *p<0.0001; scale bars=50 μm; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; L, lens; ON, optic nerve; dpf, days post fertilization; MO, morpholino).

Expression Data

Gene:	EGFP
Antibody:	Ab-4C12
Fish:	ucd1Tg ucd1Tg + MO3-insm1a
Knockdown Reagent:	MO3-insm1a
Anatomical Terms:	retinal cone cell retinal rod cell
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-insm1a
Knockdown Reagent:	MO3-insm1a
Observed In:	retinal cone cell retinal cone cell differentiation retinal rod cell retinal rod cell differentiation
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 380(2), Forbes-Osborne, M.A., Wilson, S.G., and Morris, A.C., Insulinoma-associated 1a (Insm1a) is required for photoreceptor differentiation in the zebrafish retina, 157-171, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.