FIGURE

Fig. 4

ID

ZDB-FIG-130815-4

Publication

Qian et al., 2013 - The DMAP interaction domain of UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase is a substrate recognition module

Other Figures

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Fig. 4

The K732N mutant fails to rescue MLII zebrafish. (A) Embryos were stained at 4 dpf with Alcian blue to reveal cartilage; representative images are shown. The percentage of embryos with a staining pattern consistent with the images shown is presented. Results represent an average of 5 individual experiments, with 30–40 embryos scored per experiment. (B) Cathepsin K activity was measured in embryo lysates, using a fluorogenic peptide substrate. Average activity from 8 to 9 independent experiments is shown. Error bars represent SD for all of the graphs. ***P < 0.01. (C) GlcNAc-1-phosphotransferase activity toward αMM was determined in embryo lysates, using the UDP-[³H]GlcNAc substrate. Results represent the average of 3 independent runs. **P < 0.05. (D) Bars represent the percentage of β-galactosidase activity that is Man-6-P modified in embryo lysates that were fractionated using a CI-MPR affinity column. Averages from 4–5 individual experiments are shown. **P < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-gnptab
Knockdown Reagent:	MO2-gnptab
Observed In:	cysteine-type peptidase activity mandibular arch skeleton
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA