six1b expression in Pax7+ cells is independent of MyoD and Myf5. (A) Cross-section of uninjected control embryos and myoD/myf5 morphant embryos at 24hpf showing mRNA expression of six1b (red) together with slow muscle marker F59 (green) or fast muscle marker F310 (green) and of Pax7 alone. six1b expression in the Pax7+ domain remains in MyoD/Myf5 double morphants. (B) Cross-section of control and myoD/myf5 morphant six1b:GFP embryos at 24hpf showing expression of Pax7 (red) together with six1b:GFP (green). six1b:GFP and Pax7 expression domains coincide in the myoD/myf5 morphants. Cross-sections were made at somite number 10. Scale bars: 50μm.
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