FIGURE

Fig. 1

ID

ZDB-FIG-130515-7

Publication

Riley et al., 2010 - Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division

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Fig. 1

General phenotype of harpy mutants. A,B: Live wild-type (WT) and mutant embryos at the 10 somite stage. Double sided arrows indicate the distinctive nose–tail bud separation in the mutants. C: Side views of live wild-type and mutant embryos at 24 hr. D,E: Anti-HuC (HU) stained neurons at 16 hr in wild-type and mutant embryos. The trigeminal ganglia (tg) are indicated, and double sided arrows indicate the width of the neural keel. Small arrows indicate single cells; note the larger size of the mutant cells. F,G: The 42,6-diamidine-2-phenylidole-dihydrochloride (DAPI) staining at 20 hr in wild-type and mutant embryos. F2 and G2 are whole embryo dissociations. Note the cell in mitosis in the WT field; no cells are found in mitosis in the mutants. H: Rca1/emi1 coding region showing individual protein domains and the locations of point mutations (asterisks) found in hrp^ti245 and hrp^x1 mutants. Scale bar = 50 μm in D,E, 5 μm in F2,G2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbxo5^ti245/ti245 fbxo5^x1/x1
Observed In:	anterior neural tube cell cycle cell division cytokinesis neural plate neuron whole organism whole organism anterior-posterior axis whole organism nucleus
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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