Fig. S9

Posterior hypothalamic wnt8b, fgf8 and fgf3 expression domains are present in embryos that lack Otp or Foxb1.2 activity. (A-C,E-K) One-dpf wild-type embryos (A,E,H), otpa^m866 embryos injected with 4 ng otpb MO (B,F,I), embryos injected with 8 ng foxb1.2 MO (C,G,J) and with 4 ng fezf2 MO (K) were subjected to WISH to detect the expression of wnt8b, fgf8 and fgf3. (A-C) The wnt8b expression domain in the posterior hypothalamus (arrows) is not changed in embryos that lack Otp (B; n=15/15) or Foxb1.2 (C; n=35/35). (E-G) The fgf8 expression domain in the posterior hypothalamus (arrows) is still present in embryos that lack Otp (F; n=12/12) or Foxb1.2 (G; n=34/34) function. (H-K) The fgf3 expression domain (arrows) is found in embryos with reduced Otp (I; n=8/8), Foxb1.2 (J; n=54/54) or Fezf2 activity (K; n=59/59). (D) The ratio of the hypothalamic (arrows) to the telencephalic (arrowhead) wnt8b expression domains (using ImageJ). The number of analyzed embryos is indicated within the bars. P=0.1364, P=0.7639 (Student’s t-test); n.s., not significant. (L-L0) Whole-mount two-color FISH to detect the expression of fgf3 and nkx2.1a in 1-dpf wild-type embryos. fgf3 is co-expressed with the hypothalamic nkx2.1a. Lateral views are shown. Tel, telencephalon; Hyp, hypothalamus; LOF, loss-of-function. Scale bar: 100 μm in A-C,E-K; 60 μm in L-L′′.