FIGURE

Fig. 6

ID

ZDB-FIG-130423-42

Publication

Tay et al., 2013 - Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle

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Fig. 6

Lgl2 and Rab11a control E-cadherin accumulation at KV cell junctions. (A-D) Optical sections showing E-cadherin immunostaining in KV cells of Tg(dusp6:memGFP) embryos. GFP (green arrows) and E-cadherin (red arrows) accumulate at lateral membranes in control (A), Rab11a MO^low (B) and Lgl2 MO^low (C) embryos. E-cadherin membrane staining was reduced in Rab11a MO^low + Lgl2 MO^low embryos (D). Scale bars: 20 μm. (E) Quantification of E-cadherin staining along lateral KV membranes. Average fluorescence intensities from multiple experiments were normalized to controls. Error bars indicate s.d. n, number of embryos analyzed. ^*P<0.05.

Expression Data

Gene:	GFP
Antibody:	Ab2-cdh1
Fish:	pt19Tg pt19Tg + MO1-llgl2 pt19Tg + MO1-rab11a pt19Tg + MO1-llgl2 + MO1-rab11a
Knockdown Reagents:	MO1-llgl2 MO1-rab11a
Anatomical Term:	Kupffer's vesicle basolateral plasma membrane
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pt19Tg + MO1-llgl2 pt19Tg + MO1-rab11a pt19Tg + MO1-llgl2 + MO1-rab11a
Knockdown Reagents:	MO1-llgl2 MO1-rab11a
Observed In:	Kupffer's vesicle basolateral plasma membrane
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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