Photoreceptor development and opsin transport in Sec13 morphant eyes. 3D opacity view of control (A,B) and Sec13 MO (C,D) stained for cones (zpr-1, A,C) and opsin (B,D) (green), phalloidin (red) and DAPI (blue). Arrowhead: dotted opsin localisation in Sec13 MO. Scale bars: 10μm. TEM of control (E) and Sec13 MO (F), see insets for additional examples. Stars: outer segments; E: ER; G: Golgi; arrowheads: dilated ER and Golgi. Scale bars: 500nm. Immunogold-labelling of opsin. Control (G) and Sec13MO (H). Arrowhead: accumulation of opsin in cell periphery. Stars: opsin label in outer segments. Scale bars: 500nm. Immunolocalisation of syntaxin-3 in control (I) and Sec13 MO (J). Arrowheads: syntaxin-3 label at plasma membrane. Scale bars: 10μm.
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