Fig. 5

Tmem88a and trim2a morphants have reduced erythrocytes and myeloid cells as shown by O-dianisidine and peroxidase staining respectively at 48 h post fertilisation. In control embryos erythrocytes are present in axial vessels and returning to the heart across the yolk. Myeloid cells are found across the yolk and randomly around the remaining parts of the embryos. There is loss of erythrocytes and myeloid cells in both trim2a and tmem88a morphants (translation and splice blocking morpholinos) without any defect in vascular development. Panel row 1 shows representative brightfield images, row 2 epifluorescent images of Tg(fli1a:egfp) embryos, rows 3 and 4 show embryos post O-dianisidine staining and row 5 show embryos post peroxidase staining. All images are lateral views with anterior to left with the exception of row 4 which are ventral views.