FIGURE

Fig. 6

ID

ZDB-FIG-130401-19

Publication

Seredick et al., 2012 - Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics

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Fig. 6

Mnx proteins maintain the late phase of Islet1 expression in MiPs independently of Nkx6. (A-D) Single spinal hemisegments of control and mnx MO-injected embryos. MiPs (closed arrowheads) and CaPs (open arrowheads) are indicated in each of the single channel panels; right column shows merged channels. (A) At 17 hpf, MiPs in control embryos strongly express Islet and Nkx6 (17/30 Nkx6⁺ MiPs in seven embryos). CaPs strongly express Islet but weakly express Nkx6. (B) MiPs in mnx MO-injected embryos continue to strongly express Islet and Nkx6 (13/22 Nkx6⁺ MiPs in five embryos). (C) At 21 hpf in Tg(nrp1a:GFP) control embryos, both MiPs and CaPs express Islet (32/32 Islet⁺ CaPs, and 31/32 Islet⁺ in five embryos. (D) In mnx MO-injected Tg(nrp1a:GFP) embryos, CaPs (60/60 Islet⁺ CaPs in 10 embryos) but not MiPs (19/60 Islet⁺ MiPs in 10 embryos), strongly express Islet. Scale bar: 20 μm.

Expression Data

Genes:	GFP isl1a
Antibody:	Ab2-nkx6
Fish:	WT js12Tg WT + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1
Knockdown Reagents:	MO1-mnx2a MO1-mnx2b MO5-mnx1
Anatomical Terms:	CaP motoneuron MiP motor neuron primary motor neuron
Stage Range:	14-19 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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