FIGURE

Fig. 1

ID

ZDB-FIG-130320-27

Publication

Choi et al., 2013 - In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration

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Fig. 1

FUCCI zebrafish for visualizing cardiomyocyte proliferation. (A) Representative maximum intensity projection of the heart of a 4 dpf cmlc2:FUCCI transgenic larva, visualizing non-proliferating (cmlc2:mCherry-zCdt1, red) and proliferating (cmlc2:Venus-hGeminin, green) cardiomyocytes, as depicted in the schematic at the top. Cardiomyocytes with only the Venus-hGeminin signal (arrowheads) were considered to be proliferating. DAPI, blue. Scale bars: 50 μm. (B) Time course of total (red⁺ and green⁺) and proliferating (green⁺) cardiomyocytes from 2 to 6 dpf. Percentages of proliferating cardiomyocytes are indicated above bars. Data are represented as mean±s.e.m. n=13-24 embryos per stage.

Expression Data

Genes:	mCherry Venus
Fish:	pd57Tg/+; pd58Tg/+
Anatomical Term:	cardiac muscle cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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