Fig. S3
csrnp1a knockdown does not affect blood flow. Morphant embryos were incubated in the vasodilator SNAP, and its ability to rescue the reduction of blood cells was analyzed (A, B, C). csrnp1a knockdown phenotype was not rescue by SNAP treatment. (A) Control 100% n = 53; (B) Mo csrnp1a atg 36,8% n = 21/57 of morphant phenotype; (C) Mocsrnp1a atg incubated with SNAP 33,9% n = 21/62 of morphant phenotype. In situ hybridizations against ventricular (vmhc) (D, E) and auricular (amhc) (F, G) markers show that injected embryos have normal heart development (100%; n = 43; n = 45 respectively). All are lateral views, anterior to the left. (H) Chart depicting the number of heartbeat in 10 seconds in control and csrnp1a morphant embryos. |