FIGURE

Fig. 1

ID

ZDB-FIG-130304-12

Publication

Lackner et al., 2013 - Segmental assembly of fibronectin matrix requires rap1b and integrin alpha5

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Loss of rap1b synergizes with itgα5, resulting in a disruption of all somite borders and abrogation of Fibronectin matrix assembly. A–F: Lateral view DIC images showing clear somite borders for (A) wild-type, (B) rap1b MO1-injected (8 experiments, 83.7% affected, n = 341), and (C) rap1b DN mRNA-injected (3 experiments, 100% affected, n = 177) embryos. For synergy experiments, rap1b MO1 or rap1b DN was injected into the progeny of heterozygous itgα5^+/- crosses. Therefore, 100% penetrance would result in approximately 25% of embryos exhibiting the phenotype. D: Anterior somite borders are disrupted in the itgα5^-/- mutant, while (E) itgα5^-/-; rap1b MO1 (9 experiments, 27.4% affected, n = 865) and (F) itgα5^-/-; rap1b DN (6 experiments, 22.3% affected, n = 455) embryos lack somite borders along the entire AP axis. (G- L) Dorsal view and (G′-L′) higher magnification dorsal view of FN localization showing clear FN matrix surrounding somites in (G–G′) wild-type, (H–H′) rap1b MO1, and (I–I′) rap1b DN embryos. J,J′: FN matrix surrounds only posterior somites in itgα5^-/- mutants, but few fibrils are seen within the paraxial mesoderm of either (K,K′) itgα5^-/-; rap1b MO1 or (L,L′) itgα5^-/-; rap1b DN embryos. For all images, anterior is to the left and embryos are at the 12–15-somite stage.

Expression Data

Antibody:	Ab2-fn
Fish:	WT itga5^tbfe1/tbfe1 itga5^tbfe2/tbfe2 WT + MO1-rap1b itga5^tbfe1/tbfe1 + MO1-rap1b itga5^tbfe2/tbfe2 + MO1-rap1b
Knockdown Reagent:	MO1-rap1b
Anatomical Terms:	notochord paraxial mesoderm somite somite anatomical region
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	itga5^tbfe1/tbfe1 itga5^tbfe2/tbfe2 WT + MO1-rap1b itga5^tbfe1/tbfe1 + MO1-rap1b itga5^tbfe2/tbfe2 + MO1-rap1b
Knockdown Reagent:	MO1-rap1b
Observed In:	caudal fin cell somite somite border tail bud dorsal side whole organism whole organism anterior-posterior axis
Stage Range:	10-13 somites to 14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.