FIGURE

Fig. 7

ID

ZDB-FIG-130228-24

Publication

Mo et al., 2012 - Apical Targeting and Endocytosis of the Sialomucin Endolyn are Essential for Establishment of Zebrafish Pronephric Kidney Function

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Fig. 7

Pronephric kidney function is disrupted in endolyn morphants. (A) 1ng of 10kDa rhodamine-dextran was injected into the common cardinal vein of control (Ctrl) or endolyn (End) morphants at 48 hpf. Images were acquired under identical conditions at 1, 5 and 24h post-injection. (B) The loss of fluorescence over time near the common cardinal vein (regions marked by white boxes in A) was quantified, normalized to the initial fluorescence observed at 1h, and plotted. Three independent injections were quantified with at least ten Class I and control embryos injected in each experiment. At 24h, retained fluorescence is significantly greater in endolyn morphants compared with controls (*P = 0.001 by Mann–Whitney rank sum test). (C) Imaging of the pronephric proximal tubule 24h after injection of 70kDa rhodamine-dextran demonstrates impaired endocytosis in endolyn morphants. Bottom panels show enlarged regions of the areas delineated by white rectangles in the corresponding upper panels. Arrowheads point to examples of endocytic vesicles in control larvae.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-cd164
Knockdown Reagent:	MO1-cd164
Observed In:	pronephros renal system process
Stage Range:	Prim-5 to Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.