FIGURE

Fig. 4

ID

ZDB-FIG-130218-17

Publication

Nikaido et al., 2013 - A Systematic Survey of Expression and Function of Zebrafish frizzled Genes

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Fig. 4

Morpholino-mediated knockdown of fzd3a, fzd9b and fzd10 in the sensitized background.

A–F) 1 dpf embryos. Melanisation patterns of typical embryos showing “elongated axis + no/slight necrosis” are presented here (see Table 4 for quantitation). G–L) 3 dpf embryos. All are left side views with dorsal oriented to the top. A, G) standard Control-MO, 20 ng/embryo. B, H) fzd3a-MO injected. 10 ng/embryo. C, I) fzd9b-MO injected. 15 ng/embryo. D, J) fzd10-MO injected. 5 ng/embryo. E, K) fzd3a, fzd9b, fzd10-MO = 10, 5, 2, ng/embryo, respectively. F, L) fzd3a, fzd9b, fzd10-MO = 10, 2, 2, ng/embryo, respectively. Scale bars: (A–F) 100 μm, (G–L) 500 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox10^m618/+ + MO1-fzd10 + MO1-fzd3a + MO1-fzd9b
Knockdown Reagents:	MO1-fzd3a MO1-fzd9b MO1-fzd10
Observed In:	pigmentation
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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