FIGURE

Fig. 2

ID

ZDB-FIG-130206-10

Publication

Dougherty et al., 2013 - Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis

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Fig. 2

wnt9a knockdown results in failure of maxillary extension. (A,B) wnt9a knockdown in the col2a:GFP transgenic (B) produced an ethmoid plate similar to that seen by Alcian Blue stain (inset, B). (C,D) EdU staining from 55-60 hpf revealed a proliferative front in the leading edge of the extending ethmoid plate during palatogenesis (arrowhead, C), but absence of new cell divisions in morphants (D). (E,F) TUNEL assay shows the shortened ethmoid plate (outlined) is not a result of increased cell death. (G,H) When maxillary process CNCCs were labeled by kaede photoconversion before maxillary extension (bracket, G), they failed to extend significantly by 4.5 dpf (H). All images are ventral views, anterior leftwards.

Expression Data

Genes:	EGFP Kaede mRFP
Fish:	nu12Tg vu234Tg zf393Tg nu12Tg + MO1-wnt9a vu234Tg + MO1-wnt9a zf393Tg + MO1-wnt9a
Knockdown Reagent:	MO1-wnt9a
Anatomical Term:	ethmoid cartilage
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nu12Tg + MO1-wnt9a vu234Tg + MO1-wnt9a zf393Tg + MO1-wnt9a
Knockdown Reagent:	MO1-wnt9a
Observed In:	chondrocyte embryonic neurocranium morphogenesis ethmoid cartilage ethmoid cartilage apoptotic process ethmoid cartilage chondrocyte ethmoid cartilage chondrocyte proliferation
Stage Range:	Pec-fin to Day 4

Phenotype Detail

Acknowledgments

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