Fig. 3

Reducing translation of vegfaa rescues the increase in angiogenesis caused by miR-1/206 loss of function. (A,B) Tg(fli-nGFP)^y7 zebrafish embryos injected at the one-cell stage with control MO (A) or miR-1/206 MO (B) were also injected with increasing concentrations of a translation-blocking AUG MO against vegfaa (vegfaa MO). Increasing amounts of vegfaa MO reduced ISV formation and rescued the phenotypes observed when blocking miR-1/206 function (B). (C) Quantification of the average number of nuclei per ISV in control MO-injected and miR-1/206 MO-injected embryos co-injected with increasing concentrations of vegfaa MO at 30 hpf. Mean ± s.d.; Student′s t-test.