FIGURE

Fig. 5

ID

ZDB-FIG-121210-25

Publication

Shin et al., 2012 - Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development

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Fig. 5

nf1a/nf1b mutants exhibit upregulation of pERK1/2. (A) Western blot analysis for pERK1/2 in protein lysates prepared from wild-type, nf1a^-/-; nf1b^+/+, nf1a^+/+; nf1b^-/- and nf1a^-/-; nf1b^-/- larvae (100 μg) reveals increased pERK1/2 levels in nf1a^-/-; nf1b^-/- larvae as compared with wild-type, nf1a^-/-; nf1b^+/+ and nf1a^+/+; nf1b^-/- larvae at 3 dpf. Equal loading was confirmed by stripping the membrane and reprobing for total ERK1/2. (B-Q) Transverse spinal cord sections of wild-type (B,F,J,N), nf1a^+/-; nf1b^-/- (C,G,K,O), nf1a^-/-; nf1b^+/- (D,H,L,P) and nf1a^-/-; nf1b^-/- (E,I,M,Q) larvae labeled with anti-pERK1/2 antibody (B–E, green), anti-pS6 antibody (F-I, red), or anti-HuC/D antibody (J-M, magenta) demonstrate marked upregulation of pERK1/2 in nf1a^-/-; nf1b^-/-larvae and intermediate levels of pERK1/2 in larvae harboring a single functioning nf1 allele as compared with wild-type larvae at 4 dpf (n=5 each for wild-type and mutant larvae). Scale bars: 40 μm.

Expression Data

Antibodies:	Ab1-elavl Ab4-rps6 Ab9-mapk
Fish:	WT nf1a^p301/p301 nf1b^p303/p303 nf1a^p301/+; nf1b^p303/p303 nf1a^p301/p301; nf1b^p303/+ nf1a^p301/p301; nf1b^p303/p303 (all 6)
Anatomical Terms:	spinal cord neuron spinal cord white matter whole organism
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nf1a^p301/p301 nf1b^p303/p303 nf1a^p301/p301; nf1b^p303/p303
Observed In:	ERK1 and ERK2 cascade spinal cord white matter
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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