Fig. 5
- ID
- ZDB-FIG-121116-36
- Publication
- Mackenzie et al., 2012 - Proliferative regeneration of zebrafish lateral line hair cells after different ototoxic insults
- Other Figures
- All Figure Page
- Back to All Figure Page
Flubendazole impairs division of hair cell progenitors. ET4:GFP larvae were treated at 5 dpf with neomycin or copper for 1 h and incubated in flubendazole for 24 h. Immunohistochemistry was performed for GFP and PHH3, which is upregulated during mitosis. (A) Two-way ANOVA followed by Bonferroni post-hoc analysis found significant increases in the proportion of PHH3-positive cells in neomycin- and copper-treated larvae when treated with flubendazole (*, p<0.05; ***, p<0.001), demonstrating that cell division was arrested in GFP-positive hair cell precursors. N = 8 fish per group. Error bars are + SD. (B) Example neuromast at 24 hpt from a fish treated with neomycin and flubendazole. Arrows mark PHH3-positive nuclei. |