FIGURE

Fig. S4

ID

ZDB-FIG-121101-31

Publication

Cox et al., 2012 - An essential role of variant histone h3.3 for ectomesenchyme potential of the cranial neural crest

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Fig. S4

Localization of wild-type mCherry-H3.3 within metaphase/anaphase cells of h3f3a^{db1092/db1092} embryos. Confocal images from wild-type and h3f3a^{db1092/db1092} homozygous embryos harboring the H2A.F/Z:GFP transgene and injected with mRNA encoding wild-type mCherry(m)H3.3 fusion protein. Merged images show that wild-type mH3.3 protein co-localizes with H2A.F/Z:GFP in the chromosomes of metaphase/anaphase cells (metaphase cells shown: arrowheads) in wild-type and h3f3a^{db1092/db1092} homozygotes (mutant, 21/21 cells in 3 embryos; wild-type, 15/15 cells in 3 embryos). Detailed analysis of fluorescence levels revealed no significant differences in the distribution of wild-type mH3.3 fluorescence between wild types and mutants. Scale bar = 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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