Fig. 1

Presence of full-length and splice variants of cdh23 mRNA in zebrafish larval and adult retina. A: Schematic illustrating the locations of the mutations in the two cdh23 mutant fish lines, reverse transcriptase (RT)–PCR primers, and the 52 and 32 in situ hybridization probes used in this study. For the RT–PCR primers, the red and orange arrows indicate the location of the nested primer set used to amplify cdh23 in panels B and C, while the cyan arrows indicate the primer set used in D. B: Developmental time-course showing the full-length cdh23 RT–PCR product isolated from eyes of various ages (n=3). C: Smaller RT–PCR products were also present. A shorter variant containing the transmembrane (TM) segment was amplified from 7 days postfertilization (dpf) and 6 months postfertilization (mpf) retinal transcripts (n=2). A short, soluble variant was amplified from 6 weeks postfertilization (wpf) eyes (n=2). Neither shorter variant was isolated from 3 years postfertilization (ypf) eyes (n=2). D: Using primers directed against sequence encoding the entire C-terminus, both the full-length version (containing exon 68) and the version present in the shorter variant containing the TM segment were isolated from eye or enucleated head (n=2). E: Schematics depicting the sequence and expected size of the full-length and two short forms of cdh23 isolated in (B) and (C).