FIGURE

Fig. 7

ID

ZDB-FIG-120824-19

Publication

Chen et al., 2012 - Haemodynamics-driven developmental pruning of brain vasculature in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Macrophages are not required for vessel pruning.

(A) Projected confocal images showing midbrain vasculature (red) and macrophages (green) in control MO- (left) and PU.1 MO-injected (right) Tg(kdrl:RFP,PU.1:gal4-uas-GFP) larvae at 3 dpf. The green signals in the vessels (arrowheads) were originated from non-specific expression of GFP in blood cells. (B and C) Representative of serial images showing vessel pruning (arrows) in a control MO- (B) and PU.1 MO-injected (C) larvae. (D) Summary of PU.1 knockdown effects on macrophage development and vessel pruning occurrence in the zebrafish midbrain. The data were obtained from 16 larvae in each group. Scales, 50 μm in (A) and 10 μm in (B). n.s., no significance; *** p<0.001 (Student′s t test). Error bars, ± SEM.

Expression Data

Genes:	EGFP RFP
Fish:	la4Tg; zf149Tg la4Tg; zf149Tg + MO1-spi1b
Knockdown Reagent:	MO1-spi1b
Anatomical Terms:	macrophage midbrain blood vasculature
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la4Tg; zf149Tg + MO1-spi1b
Knockdown Reagent:	MO1-spi1b
Observed In:	blood vessel remodeling macrophage midbrain blood vasculature
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.