Fig. 3
- ID
- ZDB-FIG-120815-47
- Publication
- Witzel et al., 2012 - The LIM Protein Ajuba Restricts the Second Heart Field Progenitor Pool by Regulating Isl1 Activity
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Ajuba Knockdown in Zebrafish Leads to an Increased Number of Cardiomyocytes at the Arterial and Venous Poles (A) Schematic representation of the Ajuba genomic locus (left) and the exons coding the three LIM domains (drawn to scale, right). The binding sites of the AUG and the splice morpholino are shown in red. (B) Control or AUG MO-injected Tg(myl7:EGFP-HsHRAS)s883 embryos at 48 hpf. Lateral views, anterior to the left, are in the top-left and bottom panels. Frontal views of the same embryos are in the middle and right panels. (C) Confocal images of control and Ajuba morphant Tg(myl7:EGFP-HsHRAS)s883/Tg(5.1myl7:nDsRed2)f2 hearts. (D) Total number of atrial cells and number of mGFP+DsRed+ and mGFP+DsRed cells in the atrium at 48 hpf. Error bars represent SD (n = 5). *p < 0.05; ***p < 0.001. (E) Confocal images of control and Ajuba AUG morphant ventricle at 48 hpf. (F) Total number of ventricle cells. p < 0.01. See also Figure S2 and Table S1. |
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| Fish: | |
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| Stage: | Long-pec |
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| Stage: | Long-pec |
Reprinted from Developmental Cell, 23(1), Witzel, H.R., Jungblut, B., Choe, C.P., Crump, J.G., Braun, T., and Dobreva, G., The LIM Protein Ajuba Restricts the Second Heart Field Progenitor Pool by Regulating Isl1 Activity, 58-70, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell