Fig. 4
- ID
- ZDB-FIG-120810-19
- Publication
- Goudarzi et al., 2012 - Identification and Regulation of a Molecular Module for Bleb-Based Cell Motility
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Motility Reprogramming of PGCs and Somatic Cells(A) dnd MO treated PGCs (green) are immotile with respect to somatic cells (red).(B) Bleb formation in immotile dnd MO treated PGCs overexpressing MLCK.(C) Motility of dnd MO-treated PGCs expressing MLCK and a dominant-negative E-cadherin.(D) Enhanced motility of dnd MO-treated PGCs knocked down for Annexin A5b in addition to the manipulation in (C).(E and F) Dnd-depleted PGCs treated with p53 morpholino (E). Tracking a nucleus of such a round PGC (blue track) reveals no movement relative to somatic cells (light blue, green, and red). Such cells treated with a morpholino mixture (F), show active migration (red and light blue tracks for PGCs, green and blue for somatic cells) and cell shape changes.Asterisks mark a stationary reference point in the soma. Arrowheads point at bleb formation positions. Arrows indicate direction of movement.See also Figure S3 and Movies S4 and S5. |
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Reprinted from Developmental Cell, 23(1), Goudarzi, M., Banisch, T.U., Mobin, M.B., Maghelli, N., Tarbashevich, K., Strate, I., van den Berg, J., Blaser, H., Bandemer, S., Paluch, E., Bakkers, J., Toli-Nørrelykke, I.M., and Raz, E., Identification and Regulation of a Molecular Module for Bleb-Based Cell Motility, 210-218, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell