FIGURE

Fig. 2

ID

ZDB-FIG-120810-10

Publication

Lee et al., 2012 - Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos

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Fig. 2

Expression of GFP in zebrafish embryos in the transient expression assay using the mixture of the plasmid pERE-Gal4ff containing theTol2transposase mRNA and the plasmid pUAS-GFP. Fertilised zebrafish eggs were microinjected at the 1–2 cell stages with the mixture of two plasmids. Injected embryos were then exposed for up to 72 hour post-fertilisation (hpf) to 17α-ethinyloestradiol (EE₂). These embryos showed specific mosaic expression of the reporter. The injected embryos exposed to 1000, 100 and 10 ng/L of EE₂ expressed GFP in cells most strongly in the somite muscle, heart and liver (72 hpf). This experiment was run in duplicate and was repeated at least seven times.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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