FIGURE

Fig. 3

ID

ZDB-FIG-120717-33

Publication

Oehlers et al., 2012 - Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis

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Fig. 3

The mucosecretory phenotype is microbiota-dependent but independent of neutrophilic inflammation. (A) Live imaging of neutrophil distribution in Tg(mpx:EGFP)ⁱ¹¹⁴ larvae co-treated with dexamethasone and DSS, and enumeration of intestinal neutrophils (n≥23 per group; two biological replicates). Scale bar: 1 mm. Error bars represent s.e.m.; ***P<0.0001 as determined by ANOVA. (B) Whole-mount larvae co-treated with dexamethasone and DSS, stained with alcian blue. Blue arrows indicate intestinal bulb staining; red arrows indicate cartilage staining. Scale bar: 1 mm. (C) Longitudinal sections of intestinal bulb from larvae co-treated with a cocktail of broad-spectrum antibiotics and DSS, stained with alcian blue. Scale bar: 50 μm.

Expression Data

Gene:	GFP
Fish:	i114Tg
Conditions:	chemical treatment: drug chemical treatment: drug chemical treatment: pharmaceutical
Anatomical Term:	neutrophil
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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